中文版
English产品类别:
酶
生化诊断:
比色法
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产品描述
规格
| Appearance | white to light yellow lyophilizate |
| Activity | ≧15.0 U/mg |
| Contaminants | Catalase ≦0.5 U/U％ |
| Stabilizer | sucrose |
| Storage condition | below-20℃ |
特性
| Molecular weight | ca. 900 kDa (gel filtration) |
| Structure | 57 kDa (SDS-PAGE) |
| Isoelectric point | 6.5 |
| Michaelis constant | 1.5×10-2M (L-glutamate) 1.3×10-4M (ammonia) 8.7×10-4M (ATP) |
| pH Optimum | ca. 7.0 |
| pH Stability | 6.5–9.5 |
| Optimum temperature | 60℃ |
| Thermal stability | below 40℃ |
| Inhibitors | methionine sulfoximine, carbamyl phosphate |
| Activators | Mg2+，Mn2+ |
| Specificity | L-glutamate (100), D-glutamate (0.8) NH₃(100), NH₂OH (12) ATP (100), GTP (2.5) |
应用
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
参考文献
Ebner, E. et al., “Methods in Enzymology,” Vol. 17A, Academic Press, New York, 1970, pp. 910–922.
