61222
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- 中文名称: 谷氨酰胺合成酶
- 英文名称: Glutamine Synthetase
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
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- 产品类别: 酶
- 生化诊断: 比色法
规格
| Appearance | white to light yellow lyophilizate |
| Activity | ≧15.0 U/mg |
| Contaminants | Catalase ≦0.5 U/U％ |
| Stabilizer | sucrose |
| Storage condition | below-20℃ |
特性
| Molecular weight | ca. 900 kDa (gel filtration) |
| Structure | 57 kDa (SDS-PAGE) |
| Isoelectric point | 6.5 |
| Michaelis constant | 1.5×10-2M (L-glutamate) 1.3×10-4M (ammonia) 8.7×10-4M (ATP) |
| pH Optimum | ca. 7.0 |
| pH Stability | 6.5–9.5 |
| Optimum temperature | 60℃ |
| Thermal stability | below 40℃ |
| Inhibitors | methionine sulfoximine, carbamyl phosphate |
| Activators | Mg2+，Mn2+ |
| Specificity | L-glutamate (100), D-glutamate (0.8) NH₃(100), NH₂OH (12) ATP (100), GTP (2.5) |
应用
The enzyme is useful for the determination of ammonia and ATP in clinical analysis.
参考文献
Ebner, E. et al., “Methods in Enzymology,” Vol. 17A, Academic Press, New York, 1970, pp. 910–922.
