61170
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- 中文名称: 乳酸脱氢酶
- 英文名称: Lactate Dehydrogenase
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
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- 应 用: 生化诊断
- 产品类别: 酶
- 生化诊断: 比色法
规格
| Appearance | white lyophilizate |
| Activity | ≧150 U/mg |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 115 kDa (gel filtration) |
| Michaelis constant | 2.5×10½M (lactate) 1.0×10¼M (pyruvate) |
| pH Optimum | 7.4–8.0 |
| pH Stability | 7.0–7.5 |
| Optimum temperature | 50–55℃ |
| Thermal stability | below 45℃ |
应用
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
参考文献
Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
