60273
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- 中文名称: 果糖基-氨基酸氧化酶
- 英文名称: Fructosyl-amino Acid Oxidase
The enzyme is useful for the determination of fructosyl-L-amino acid.
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- 应 用: 生化诊断
- 产品类别: 酶
- 生化诊断: 比色法
规格
| Appearance | yellow lyophilizate |
| Activity | ≧4.0 U/mg |
| Stabilizer | trehalose |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 45 kDa (gel filtration) |
| Structure | monomer of 50 kDa (SDS-PAGE) |
| Michaelis constant | 2.2×10-4M (Nε-fructosyl-L-lysine) |
| pH Optimum | 8.0–8.5 |
| pH Stability | 6.0–8.5 |
| Optimum temperature | 35–40℃ |
| Thermal stability | below 30℃ |
| Stability (liquid form) | stable at 25℃ for at least two weeks |
| Stability (powder form) | stable at 37℃ for at least three weeks |
| Inhibitors | Ag+，Cu2+ |
| Specificity | Nε-fructosyl-L-lysine (100), fructosyl-L-valine (65) |
| fructosyl-glycine (30) |
应用
The enzyme is useful for the determination of fructosyl-L-amino acid.
参考文献
Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).
Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).
Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).
