60189
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- 中文名称: β-磷酸葡萄糖变位酶
- 英文名称: Beta-Phosphoglucomutase
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
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- 应 用: 生化诊断
- 产品类别: 酶
- 生化诊断: 比色法
规格
| Appearance | white lyophilizate |
| Activity | ≧30 U/mg |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA) |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 34 kDa (gel filtration) |
| Structure | monomer of ca. 25 kDa (SDS-PAGE) |
| Michaelis constant | 2.3×10-4M (β-D-glucose-1-phosphate) |
| pH Optimum | ca. 7.0 |
| pH Stability | 5.0–9.5 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at lest one month |
| Activators | Mg2+，Mn2+，Co2+，Ni2+ |
| Inhibitors |
Hg2+，Zn2+，Cu2+，Cd2+ |
应用
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
参考文献
Nakamura, K. et al., J. Ferment. Bioeng., 85, 350–353 (1998).
Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).
