60122
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- 中文名称: 蔗糖磷酸化酶
- 英文名称: Sucrose Phosphorylase
The enzyme is useful for the determination of inorganic phosphate in clinical analysis.
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- 应 用: 生化诊断
- 产品类别: 酶
- 生化诊断: 比色法
规格
| Appearance | white lyophilizate |
| Activity | ≧50 U/mg |
| Stabilizer | sucrose |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 56 kDa (gel filtration) |
| Structure | monomer of 56 kDa (SDS-PAGE) |
| Isoelectric point | 4.6 |
| Michaelis constant | 3.9×10-2M (sucrose) |
| 6.2×10-3M (phosphate) | |
| pH Optimum | 7.5 |
| pH Stability | 5.0–8.0 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least two weeks |
| Inhibitors | glucose, glucono-1,5-lactone |
| Specificity | sucrose (100), maltose (0), starch (0) |
应用
The enzyme is useful for the determination of inorganic phosphate in clinical analysis.
参考文献
Koga, T. et al., Agric. Biol. Chem., 55, 1805–1810 (1991).
Kitao, S. and Nakano, E., J. Ferment. Bioeng., 73, 179–184 (1992).
